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How can i know the flow cell in FLD is contaminated

http://www.chromforum.org/viewtopic.php?t=24690

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How can i know the flow cell in FLD is contaminated

Posted: Sat Apr 26, 2014 7:27 am
by ranjithmuthoju
Hi,
how can i know the flow cell in the Flourescence detector is contaminated , if there is any problem in flow ,can effect the samples results ,if so how can i clean the flow cell ..?

Re: How can i know the flow cell in FLD is contaminated

Posted: Sat Apr 26, 2014 9:44 am
by uzman
Take a Raman spectrum of pure water , and check the S/N ratio according to device specifications.

You may clean the flow cell by passing first water , then IPA.
After cleaning with IPA , pass water again before proceeding with mobile phase ( if it is reverse phase method )
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