HMDS-fatty acids

[GCrF]

Dear all,

can someone suggest me a sylation protocol using the HMDS reagent?

Many thanks

[MSCHemist]

Are we talking short chain fatty acids? Otherwise most labs do FAMES. I prefer chloroformates over silytating reagents since they are not water sensitive and don't foul up the instrument.

http://onlinelibrary.wiley.com/doi/10.1 ... 3/abstract

[GCrF]

Are we talking short chain fatty acids? Otherwise most labs do FAMES. I prefer chloroformates over silytating reagents since they are not water sensitive and don't foul up the instrument.

http://onlinelibrary.wiley.com/doi/10.1 ... 3/abstract

with FAMEs method I've some difficulties to detect hydroxy-F.A. I'm interested on C-12 to 18.

[MSCHemist]

HMDS is a rather weak agent.

From Handbook of Analytical Derivitizations
Dissolve in HMDS/TMCS/pyridine 2:1:10 add 2.5 molar equivalents BSA in pyridine and incubate at RT for 2 hours

or
dissolve in 50ul BSTFA, 50ul Pyridine heat to 50-60 deg for several hours [I'd imagine inlcuding 1% TMCS would help]