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injection of a mobile phase in gradient elution of hplc

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injection of a mobile phase in gradient elution of hplc

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M191
My mobile phase and sample injection has common peak but 100% diluent inection has no peak .need clarification any help please
:alien:

Re: injection of a mobile phase in gradient elution of hplc

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tom jupille
Site Admin
Details, details, details! We need details.
Column? Mobile phase? Diluent? Detector (and wavelength, if UV)? Isocratic or gradient?
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
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