i tried to calculate the Dissolution without Dissolution addon software from waters
Calculation:


1. Standard Concentration Calculation (Concentration of drug).

mg/mL

2. drug Concentration is individual Vessel.

mg/mL

3. Amount of drug in individual Vessel at specific time point.


Sampling Time (Hr) (t) Time point No. (n) Amount of drug at specific time point (X)
0.25 1 Cn x [900-(n-l)xV]
0.50 2 Cn x [900-(n-l)xV] + V x (C1)
0.75 3 Cn x [900-(n-l)xV] + V x (C1+C2)
1.00 4 Cn x [900-(n-l)xV] + V x (C1+C2+C3)
2.00 5 Cn x [900-(n-l)xV] + V x (C1+C2+C3+C4)
2.25 6 Cn x [1000-(n-l)xV] + V x (C1+C2+C3+C4+C5)
2.50 7 Cn x [1000-(n-l)xV] + V x (C1+C2+C3+C4+C5+C6)
2.75 8 Cn x [1000-(n-l)xV] + V x (C1+C2+C3+C4+C5+C6+C7)
3.00 9 Cn x [1000-(n-l)xV] + V x (C1+C2+C3+C4+C5+C6+C7+C8)
4.00 10 Cn x [1000-(n-l)xV] + V x (C1+C2+C3+C4+C5+C6+C7+C8+C9)

4. % drug Dissolve at specific time point


Cstd=Standard Concentration in mg/mL
Stdwt=Weight of Standard
VStock=Volume of Stock Standard Solution used to prepare final standard solution.
P=Purity of Standard
CF=Conversion Factor
Cn=Sample Concentration at specific (nth) time point
Asample=Area of Sample
Astd=Average Area of System Suitability standard
V= Volume Removed at each time point i.e.3 mL
n= Time point number
X=Amount of drug at specific time point
LC=Label Claim


To calculate CFV1: (Correction Factor)
1V1..(Amount)+2V1..(Amount)+3V1..(Amount)+4V1..(Amount)+5V1..(Amount)+6V1..(Amount)+7V1..(Amount)+8V1..(Amount)+9V1..(Amount)+10V1..(Amount)
1V2..(Amount)+2V2..(Amount)+3V2..(Amount)+4V2..(Amount)+5V2..(Amount)+6V2..(Amount)+7V2..(Amount)+8V2..(Amount)+9V2..(Amount)+10V2..(Amount)
1V3..(Amount)+2V3..(Amount)+3V1..(Amount)+4V3..(Amount)+5V3..(Amount)+6V3..(Amount)+7V3..(Amount)+8V3..(Amount)+9V3..(Amount)+10V3..(Amount)
((Amount*Media_Volume)+(CFV2*Sample_Volume))/Label_Claim*100
((Amount*Media_Volume)+(CFV3*Sample_Volume))/Label_Claim*100

Need more support on this