Chromatography Forum

Has anyone in here worked on measuring Ginseng (marker compounds Ginsenosides) in multivitamin products?

I am starting a project to quantitate ginsenosides with hplc.

try this site: http://herbalgram.org/default.asp?c=ginsenoside
they extract from capsules and gel caps, etc.

Your 7 major standards are:
Standards: Ginsenoside Rb1 (Indofine Chemical Co., Cat. # 02-0105S) or (Sigma, Cat. # G 0777) 100% by HPLC
Ginsenoside Rb2 (Indofine Chemical Co., Cat. # 02-0104S) 100% by HPLC
Ginsenoside Rc (Indofine Chemical Co., Cat. # 02-0106S) or (Sigma, Cat. # G 0902) 99% by HPLC
Ginsenoside Rd (Indofine Chemical Co., Cat. # 02-0102S) 100% by HPLC
Ginsenoside Re (Indofine Chemical Co., Cat. # 02-0103S) or (Sigma Cat. # G 1027) 99.7% by HPLC
Ginsenoside Rg1 (Indofine Chemical Co., Cat. # 02-0101S) 100% by HPLC
Ginsenoside Rf (Indofine Chemical Co., Cat. # 02-0107S) 100% by HPLC


a perfectly good (but long) hplc program is

Chromatographic Conditions

Column: YMC ODS-A, 3µm, 3 x 150 mm, 120 Å, or equivalent (C18, 17% carbon loading, end-capped)
Column Temperature: 40°C
Mobile Phase: gradient

Time (minutes) % Acetonitrile % Water
0 20 80
22 22 78
40 50 50
50 55 45
52 65 35
54 20 80
70 20 80


Flow Rate: 0.6 mL/min,
Injection Volume: 12µL
Detection: 205 nm
Run Time: 70 minutes

Vestel,

Sorry it has take me so long to get back to you.

Here is the gradient:


Columns: 2 - EMerck Chromolith 4.6x100mm columns C18
3mL/min A=Water B=Acetonitrile
(T(min), %B) (0, 20) (4, 20) (17, 40) (17.5, 40) (17.51, 20)

Collect data for 17 minutes equilibrate for 4-5 minutes.

Sample prep is critical

Use Waters Oasis HLB Cartridges - acts like reverse phase.

3cc 60mg

Extract 200-600 mg of 4-5% w/w ginsenosides extract with 40mL of MeOH, mix MeOH extract to 30% with water, filter thru 0.45mcm filter, condition HLB cartridge with 1mL MeOH, 1mL H2O, Add 12 mL of 30% MeOH sample, rinse with 1-3mL of 30% MeOH with 2% NH4OH, and/or 1-3mL of 30% MeOH with 2% Acetic Acid, air dry the cartridge, finally elute with 1mL of MeOH. The NH4OH is used to elute the acidic interferences. The acetic acid is use to remove the basic interferences. This also has the ability to concentrate the samples.

I detect using ELSD, but if your sample is concentrated enough, 200nm UV will also work.