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Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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Hello, I'm new in this forum and at HPLC method too.
I use a REZEX ROA H+ column for Organic Acid, at 75°C using H2SO4 like eluent.
Normally a run is 40min and I can see Lattic A., Succinic A., Acetic A., Formic A., Propionic A., Isobutirric A. Butirric, Isovaleric A. and Valeric A.
Friday I try a run of 90 minutes and I found a new peak at 47 min.
question is: Someone know what It could be? or How can I know what it could be?
Thanks a lot
Could be almost anything: another organic acid, an alcohol, etc. Best place to start is with knowledge about the chemistry of your sample (i.e, what is likely to be in there).
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
2 posts Page 1 of 1

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