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can use ammonium chloride in LCMSMS-ESI-?
Posted: Wed Feb 19, 2014 3:19 am
by hoangduc
We are developing method for determining a drug compound in human plasma by lcmsms. We considered some adduct solution. Result: with ammonium chloride as adduct (-) intensity is maximum. However, i don't know Can ammonium chloride cause corrosion problem?
Re: can use ammonium chloride in LCMSMS-ESI-?
Posted: Wed Feb 19, 2014 5:18 pm
by JMB
The Cl anion is notoriously corrosive to stainless steel, and it is usually recommended by HPLC manufacturers to either avoid it or flush the HPLC pumps/lines very thoroughly after use.
If you can set up the runs to include such flushing at the end of the batch, then you will be OK.
The ESI-MS source should not be affected, but as a guess (I have not run multiple LC/MS with Cl anion present) the capillary may age a little faster than usual.
Re: can use ammonium chloride in LCMSMS-ESI-?
Posted: Sat Feb 22, 2014 2:00 am
by gapeev
I used NH4CL as an HPLC additive at about 1 ppm concentration for LC-MS ESI(-) runs. Even published some papers. No problems noticed. And yet, Cl(-) won't do no good to your LC. We HAD to use it to get all the analytes s into the [X]Cl- form. Cl(-) is not a LC friend for sure.
Where it boils down to is that using little of PURE NH4Cl followed by water rinse at the end of a batch won't hurt.