Chromatography Forum

Hello, since the engineer hasn't come up with any useful suggestions yet I thought someone here may have experienced the same problem (and perhaps solved it).
We have an Agilent 7683 injector and were using Chemstation E.01.01 so far and everything was working fine. Now we had to have version E.02.02 installed to make it compatible with Windows7 and since then the autosampler is doing something rather weird. Injecting is fine but when it rinses the 50 uL syringe with solvent it pulls the plunger up to about 15 uL, takes it out of the solvent vial, pulls the plunger up all the way and then dispenses it to waste. I could imagine that this is the "solvent saver" mode but this option it is not ticked (and I have also ticked it, saved it, unticked it, saved it again) and I don't want to save solvent. I read somewhere that this solvent saver option is actually only available from 7683 B series. Could this be the reason why it gets confused?
Many thanks for any helpful advice (or some commiseration at least)!

You will actually get more effective syringe washing using multiple small quantities than one or two fills to full capacity.

Have you checked that the syringe volume is set correctly ?

Peter

I'm using 12 washes in total, 6 from each of two vials and would just like to have it work the same way again since I know that there was no carry-over. The syringe volume is set correctly (also in the "Consumables Panel") and it does injections using the right volumes. Just keeps having its own mind when it comes to rinsing...