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conversion of Gradient run to isocratic run

http://www.chromforum.org/viewtopic.php?t=24260

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conversion of Gradient run to isocratic run

Posted: Thu Feb 13, 2014 6:20 am
by saipraveen7
I have run a Gradient program on HPLC and the peaks were well resolved. Now I would like to change the gradient program to isocratic. Is there any simple formulae for converting gradient run to isocratic run.

- P.Sai Praveen

Re: conversion of Gradient run to isocratic run

Posted: Thu Feb 13, 2014 1:00 pm
by Gerhard Kratz
Increase %B by 30% to start with your isocratic test run. You will need some trials.
Good luck

Re: conversion of Gradient run to isocratic run

Posted: Thu Feb 13, 2014 1:37 pm
by tom jupille
http://chromforum.org/viewtopic.php?f=31&t=19492
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