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Problem with LC analysis of Vilazodone
Posted: Tue Feb 11, 2014 11:19 am
by santoshgandhi
Presently we are working on a drug vilazodone (Antidepressant) and we are having a difficulty with LC analytical method. The peak shape obtained for drug is very broad (peak width around 2 minute) as well as tailing & fronting effect. We had numerous trials using different mobile phases like methanol: phosphate buffer pH 3 adjusted by orthophosphoric acid (80:20), Acetonitrile : methanol (90:10). Also we worked at different pH (Acidic as well as Alkaline), yet could not get sharp peak. Kindly suggest solution.
Re: Problem with LC analysis of Vilazodone
Posted: Tue Feb 11, 2014 11:26 am
by alemaggot
what is your analyte concentration?
How many ul you inject?
What column you use?
Re: Problem with LC analysis of Vilazodone
Posted: Tue Feb 11, 2014 1:48 pm
by Gerhard Kratz
Fronting is a problem in general issued by extra column effects, bad connections, dead volume etc.!
What is the pK of your drug?
Can you use also some volatile buffers, instead of phosphate buffer?
What column material you use, particle size etc.?
Dimension of your column can also play a role. Maybe another separation mode can do the job, like HILIC!
Re: Problem with LC analysis of Vilazodone
Posted: Tue Feb 11, 2014 2:30 pm
by emeulman