Desalting of Polysacharide samples

[josebenjamin]

Dear Friends,

I need to process some polysacharide samples (MW 4500) for HPLC analysis. Part of the problem is to remove salts. I have considered the use of GPC or GFC materials abvailable in cartridge form.

Do you know of any other SPE-type materials that could be used for this? The obvious need is to remove salts without any carbohydrates being lost.

Thanks,

josebenjamin

[moon704520]

you can try desalting by hplc

[HW Mueller]

There is also ultrafiltration or even dialysis.

[josebenjamin]

Dear friends,

Thanks for the comments above. What I would like to hear are some comments from people with practical experience using disposable SPE-type cartridges for sample desalting.

josebenjamin