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do we have any equation to predict temperature effect on gradient retention time?
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Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
Only in the sense that everything happens faster, so any absolute errors are smaller. Percentage errors are about the same.gradient time is more robust for steep gradient than shallow gradient, right?
Yes, but it's complicated. k* values change roughly in proportion to 1/T (in °K). Read Snyder & Dolan's book on High Performance Gradient Elution for the math (here's a link to it on Amazon: http://tinyurl.com/ct68wxz )do we have any equation to predict temperature effect on gradient retention time?
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