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temperature on gradient retention time

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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gradient time is more robust for steep gradient than shallow gradient, right?
do we have any equation to predict temperature effect on gradient retention time?
gradient time is more robust for steep gradient than shallow gradient, right?
Only in the sense that everything happens faster, so any absolute errors are smaller. Percentage errors are about the same.
do we have any equation to predict temperature effect on gradient retention time?
Yes, but it's complicated. k* values change roughly in proportion to 1/T (in °K). Read Snyder & Dolan's book on High Performance Gradient Elution for the math (here's a link to it on Amazon: http://tinyurl.com/ct68wxz )
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
2 posts Page 1 of 1

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