Acquity UPLC column compatibility with Agilent 1200 series

[pkaloudis]

Hello all,

I have a question regarding the compatibility of an Acquity UPLC CSH C18 column (150mm x 2.1mm x 1.7μm) with an Agilent 1200 series.

In the lab we have an Agilent 1200 system and for a new project we are considering ordering an Acquity UPLC CSH C18 column. When I contacted the local Waters representative they told me that this column is compatible with the Waters Acquity UPLC system and its use with other LC systems is not recommended or supported.

Does anyone has any feedback from similar situation? Will I encounter compatibility issues or they are just trying to "scare" me in order to sell the Waters Acquity UPLC system?

The flow rate proposed in the literature for this analysis is low, so I don't expect backpressure to be a problem here.

Thank you

[bisnettrj2]

Which 1200 series system do you have? If you have a UHPLC-type 1290, then you CAN use an Acquity column on your system, as backpressure, even at a low flow rate, IS going to be an issue with a 1.7um particle size. However, Waters will not guarantee the performance of the column on a system other than an Acquity. Also, you'll need to be aware that the Waters end fittings are different than Agilent column end fittings, so you'll need to make new connections to the column if you have swaged stainless steel lines on the instrument already.

If you are not using a UHPLC, then forget about this column, and instead buy something in a standard format, like this: http://www.waters.com/waters/partDetail ... =186004770 You'll get the separation you need at a flow rate and pressure your system should be able to handle.

[pkaloudis]

Which 1200 series system do you have?

I am using the 1260 Infinity series system which can handle up to 600 bar of backpressure.

Also, you'll need to be aware that the Waters end fittings are different than Agilent column end fittings, so you'll need to make new connections to the column if you have swaged stainless steel lines on the instrument already.

I have also used other Waters columns on the Agilent 1260, so the different end fittings shouldn't be a problem, but since this is the first time that I am considering sub 2μm particles, I am not really sure if the end fittings are different than the other Waters columns in order to cope with the higher backpressures.

[bisnettrj2]

The Waters end fittings are different from other manufacturers' columns. And 600 bar isn't enough backpressure capability to effectively run a 1.7um particle size column, especially at those dimensions. Maybe if you were running a 2.1X50mm, but not a 150. In any case, you need to be especially cognizant of pre- and post-column volumes, and need to keep them as low as possible, in order to achieve a good separation with that small a particle size. You might want to ask Waters if the other column I recommended is a suitable replacement for the column you want to run.

[MSCHemist]

I've run Water's UPLC columns on an Agilent 1290 before. There is an adaper with a split plastic thingy and front ferrule that you can use before the column to create the proper line protrusion ammount. I don't recall what it was called or where It came from but hopefully someone else can help you.