Hi All,

We are working on the method development for Triamterene and Hydrochlorothiazide. Hydrochlorothiazide gives very small carryover. The main problem is with Triamterene.
Based on our experiments we most probably have 2 sources of carryover, since bigger part of it disappears, when after highest standard injection we are taking away needle seat and flushing it with acetonitrile or methanol.
We also have controversial results: when we are using custom injector program which allows us to move the needle from the seat several times to evaluate the existence of drop in this part, it decreases carryover by 1/3.
We have an Agilent 1290 Infinity System coupled with AB Sciex API 4000 mass spectrometer.

We are looking forward to hear your recommendations on how to deal with this type of carryover!

Thanks in advance!