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L-Cysteine
Posted: Tue Dec 17, 2013 9:17 am
by rojitea
Could any one share HPLC condition & sample preparation for L-Cysteine in finished dosage form.
Re: L-Cysteine
Posted: Tue Dec 17, 2013 10:32 am
by Gerhard Kratz
A Little Google will help you!
Re: L-Cysteine
Posted: Sat Jan 11, 2014 9:48 pm
by ph/Amr Tarek
HPLC analysus for cysteine :
1) detector : RF 535 flurescence spectrophotometer operating excitation of 300 nm and emission of 380 nm .
2) column : 100 × 4.6 mm C18
3) mobile phase :
a) solution A : 800 ml water + 200 ml acetonitrile + 1 ml acetic acid + 1 ml phosphoric acid
b) solution B : 200 ml water + 800 ml acetonitrile + 1ml acetic acud + 1ml phosphoric acid
mix solution A with B ( 25% : 75% ) ( A : B )
4) flow rate : 0.5 ml/min
5) inj. volume : 20 micro litre
Re: L-Cysteine
Posted: Sun Jan 12, 2014 1:49 pm
by uzman
Hi pH/Amr Tarek ,
Why are you preparing the mobile phase like this ?
It can be prepared as a 350 ml water + 650 ml acetonitrile + 1 ml acetic acid + 1 ml phosphoric acid.
Re: L-Cysteine
Posted: Mon Jan 13, 2014 9:25 pm
by MSCHemist
I do cysteine with GC (FID or MSD).