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extraction of fatty acids
Posted: Sat Aug 13, 2005 11:16 am
by famahyari
I want preparation FAME with BF3 /methanol, so I should extract the fatty acids.
I want a procedure for extract fatty acids from real sample(e.g. fish oil) then estrify with BF3 /methanol.
please help me
thanks
Posted: Mon Aug 15, 2005 7:56 am
by HW Mueller
Having followed this other chain, I am inclined to say that if you were less belligerent, but more precise you would long since have an answer. Though not having worked with FA for a few years, I will try to figure what you want: Apparently you should determine the unesterified fatty acids (UEFA) in the oil? If you have an oil, someone already did an extraction, possibly missing most of the UEFA, which are most likely at a very low concentration to start with (depending on what was extracted).
I am sure the literature has sundry examples, but one can imagine that TLC or HPLC could be used to separate all lipid classes, then one could do the BF3/MeOH on the UEFA fraction. Or, methylating only the UEFA with diazomethane might be a possibility. Also, extracting the UEFA into a basic aqueous solution (should be weakest possible?) might be a possibility.
Posted: Mon Aug 15, 2005 9:41 am
by famahyari
thanks a lot
is it correct that I add directly BF3/methanol to oil.
I want to determine the omega-3 in fish oil.
Posted: Mon Aug 15, 2005 2:33 pm
by HW Mueller
It´s more like the other way around, if you want to know total omega-3.
Posted: Mon Aug 15, 2005 2:47 pm
by Consumer Products Guy
Search this forum, I've posted on this numerous times in the past.....too busy to do again....
Posted: Mon Aug 15, 2005 3:04 pm
by famahyari
thanks a lot
fish oio
Posted: Tue Aug 16, 2005 8:53 am
by nina
Hi
how can I preparation fish oil for analysis?
thanks