Chromatography Forum

Good Evening Experts,

I want to separate both of this isomers 2,4 CFT, (i.e 2-Chloro 4-Fluoro Toluene) and its isomer (4-Chloro,2 Fluoro Toluene) which condition is suitable for this????

Best regards

Praveen

I'd try to either use a PFP column, which usually shows unique selectivities for halogenated compounds, or some sort of C18 with a dense polymeric bonding (e.g. Nucleosil AB or Purospher STAR) which often are able to separate isomers because of their high steric selectivity.

You might also consider the porous graphitic carbon (e.g. Hypercarb) type column; these are advertised as very suitable for resolution of geometric isomers and diastereoisomers.

I do not have the above described columns. Should I have use Atlantis T3 which have high silanophylic activity so that there is H- bond formation between analyte and active silanols may induced some separation.
One more thing should I have to use normal phase??

Praveen

Your best bet is to try GC!

I also agree for this On HPLC I got the sufficient separation but the run time is too long. I also want to switch the method on GC but which column is suitable for this ????

Hi Praveen,

Please check out the Website of RESTEK for applications on such compounds. good luck.

Sir,

I have checked the RESTEK website but did not found any clue. Can u tell me another option regarding to this analysis.

Best regards

Praveen

Gas chromatographic determination of aromatic amines in water samples after solid-phase extraction and derivatization with iodine: I. Derivatization
Torsten C Schmidta, Corresponding author contact information,
Melanie Lessb,
Rainer Haasc,
Eberhard von Löwb,
Klaus Steinbacha,
Gottfried Storka
a Department of Chemistry, Philipps-University, Hans-Meerwein-Strasse, D-35032 Marburg, Germany
b Environmental Hygiene and Immunology, Medical Centre of Hygiene, Philipps-University, Pilgrimstein 2, D-35037 Marburg, Germany
c Bureau for Exploration of Hazardous Waste Sites and Pollution Research, Stadtwaldstrasse 45a, D-35037 Marburg, Germany
Maybe this will help.

I just check all the information above given but it is just releated to Aromatic amines but how can I got the information about the separation of CFT isomers????

Praveen

If you go to any of those sites that sell GC columns, I am sure if you talk to their technical support they will be able to point to in the right direction. Our lab typically uses restek or J&W (agilent) columns. There is probably a capillary column out there that would work for you. In the mean time I submitted a tech support request to see what they would say.

Edit: I would assume a DB-624 or DB-1 column would suit you just fine if you guys have them already.

Thanks for all of your comments. I found the separation on GC column and also found the LOD around 0.1%.
As per most of your comments I tried the analysis on GC column and found very good separation.
Thanks all of you.

Best regards

Praveen