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How to remove P-80 and PEG300 before analysing formulatin

http://www.chromforum.org/viewtopic.php?t=23890

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How to remove P-80 and PEG300 before analysing formulatin

Posted: Tue Dec 03, 2013 5:13 am
by bobby12
I have to analyze formulation using ESI mass spec. I have 2.5% Polysorbate 80 and 10% PEG in it. How do I remove them and analyze my API and impurities. I am worried it might contaminate or cause ion suppression in mass spec.

Is there a way to elute them in one or two min and divert first two min in waste?

Re: How to remove P-80 and PEG300 before analysing formulati

Posted: Fri Dec 13, 2013 2:51 pm
by JMB
You should look into Turbulent Flow Chromatography, which is used to separate unwanted matrix components from analytes.
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