Chromatography Forum

http://www.sielc.com/Application-Loadin ... raphy.html


Can someone explain what is going on here, why the peaks look the way they do at various amounts injected.

How does one assess "loadability" from this data?

You are injecting large amount of sample into analytical column, as you can see even analytical column can tolerate "semi-prep" separation. Very high capacity of the column is attributed to high capacity of the single-ligand design of the mixed-mode stationary phases. If you try anything like this on any other traditional column your polar ionizable hydrophilic compounds will not retain in most of the cases. If you will be able to achieve retention overloading of silanol site will cause your peaks move towards the void. The graph represents on how much you can load into the column. Loading capacity will also depend on how well your compounds are separated. A lot of our customers are using smaller ID columns 94.6 mm instead of 10 mm, 10 mm instead of 21.2 mm, etc.) to do prep separation.

Here is another graph on capacity:
http://www.sielc.com/Products_Obelisc.html (scroll to the bottom of the page)

Contact me if you have questions.

How would you put a "number" on loadability from that data?

You are injecting large amount of sample into analytical column, as you can see even analytical column can tolerate "semi-prep" separation. Very high capacity of the column is attributed to high capacity of the single-ligand design of stationary phase. If you try anything like this on any other traditional column your polar ionizable hydrophilic compounds will not retain in most of the cases. If you will be able to achieve retention overloading of silanol site will cause your peaks move towards the void. The graph represents on how much you can load into the column. Loading capacity will also depend on how well your compounds are separated. A lot of our customers are using smaller ID columns 94.6 mm instead of 10 mm, 10 mm instead of 21.2 mm, etc.) to do prep separation.

Here is another graph on capacity:
http://www.sielc.com/Products_Obelisc.html (scroll to the bottom of the page)

Contact me if you have questions.

Hi Vlad:

Interesting stationary phases. Have you done LSER analysis for them?

no LSER analysis on any of our stationary phases