Phosphate Buffer in LC-MS
Posted: Mon Nov 11, 2013 4:20 am
Hi,
I'm using 5mM Ammonium Phosphate, pH 7.0 (with Ammonia)/Acetonitrile = 50/50 to separate my polar compounds on a HILIC column using HPLC. Has anyone some advise how i could transfer this method to the LC-MS without clogging everything up and getting Ion Suppression? Is a concentration like that maybe even okay for use with the LC-MS?
I'd appreciate any advise on this,
many thanks!
I'm using 5mM Ammonium Phosphate, pH 7.0 (with Ammonia)/Acetonitrile = 50/50 to separate my polar compounds on a HILIC column using HPLC. Has anyone some advise how i could transfer this method to the LC-MS without clogging everything up and getting Ion Suppression? Is a concentration like that maybe even okay for use with the LC-MS?
I'd appreciate any advise on this,
many thanks!