Chromatography Forum

Hi




Any idea why this happened?
I changed the source and EM horn, baked the MS two times for 12Hrs but nothing improved

Vent and check to see if all electrical conections are correct and tight. Also check that you have PFTBA in the vial.

What is that vacuum reading??

Is your high vacuum gauge working correctly?

Do you have any idea when or why it started?
any maintenance done, column change, ..... ?

Have you tried reloading an old tune file? If your current file gets some corrupt value in it the system may not be able to find peaks. It's worth a shot.
If you haven't done it before, find an old tune file from when things were working and rename it as your method tune file (atune.u).

thanks everybody.

the situation began after AC unit dropped waters on the autosampler tray.
the vacuum is 100%, and as I said I changed the repler assembly and EM horn with new parts and refill calibration cell.
manufacturer tuning value and last good tune value used but not successful.
the strange thing is the peak width!!

thanks everybody.

the situation began after AC unit dropped waters on the autosampler tray.
the vacuum is 100%, and as I said I changed the repler assembly and EM horn with new parts and refill calibration cell.
manufacturer tuning value and last good tune value used but not successful.
the strange thing is the peak width!!

Look very closely at the side board on the analyzer!

Check for any resistors that look swollen or cracked, or any traces that look as if water hit the board. We had a similar problem where condensation fell on a 5971 and shorted the top board( same as the side board on 5975) which controls the quadrupoles. The photo looks exactly like a problem with the mass filter/quads which is why I was asking about the vacuum as it affects peak widths just as a quad driver problem does. If you have another unit you can swap that board and see if the problem goes away. But I am willing to bet that is where your problem is.

Check the calibrant , actually take the vial out and check it has liquid, sometimes it is really hard to see because of the shape of the bubble in the bottom of the vial. Also check seating of EM horn, make sure pin is in contact and on outside of copper strip. Lastly or really firstly check all electrical connections. Swap filament.

Have you try with filament #1?
If you have refill the calibration vial, it is important to not overfill the vial..see procedure in Hardware manual and don't forget to purge calibrant valve.