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UV absorbance in blank sample

http://www.chromforum.org/viewtopic.php?t=23725

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UV absorbance in blank sample

Posted: Fri Nov 01, 2013 7:53 pm
by jiangds06
in base line, it shows huge absorbance in 225 nm. How can get rid of it?

Re: UV absorbance in blank sample

Posted: Sun Nov 03, 2013 4:13 pm
by HPLCaddict
More details, please!
What do you mean by "blank sample"? is it a solvent injection or a blank run without injection?
What does "huge absorbance" mean? A single peak? Multiple peaks? t0 noise? Baseline drift?

Re: UV absorbance in blank sample

Posted: Mon Nov 04, 2013 12:26 pm
by Gerhard Kratz
What flow, what mobile Phase, what HPLC System, compound dissolved in??
Yes, we Need more Details.
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