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- Posts: 56
- Joined: Fri Mar 20, 2009 11:14 am
I am trying separate Caffeine with HPLC using a C18 column. I would like to avoid using buffers if possible but I am getting distorted peaks and even split peaks in some chromatographic conditions. I found it difficult to get good retention with good peak shape using Methanol and Water only as the mobile phase. I have an access to a C8 columns if it may help.
I appreciate your suggestions.
Many thanks in advance.