Chromatography Forum

Dear all,

Can anyone advise me whether we have to integrate the peaks eluted before the solvent peak in the sample chromatogram by HPLC. These peaks are not eluted in the blank.

Thanks in advance for any replies.

Ideally any peaks eluting before solvent peak is not to be considered for integration as the reproducibility of these peaks will not be good. Now in your case you said the same peak is not seen in blank then you should try to modify the chromatography to retain this particular peak to some considerable capacity factor.