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buffer concentration for LCMS.

http://www.chromforum.org/viewtopic.php?t=2362

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buffer concentration for LCMS.

Posted: Mon Aug 08, 2005 10:25 pm
by michelle.zhang
Dear members,
I want to transfer a LC method developed before from the LC to LCMS,
which method was developed for LC-ELSD oringinally. I successfully did it.
but I want be sure if the concentration is too high for LC Mass?
it is 0.1M NH4OAc /HOAc.
thank you.
Michelle

Posted: Mon Aug 08, 2005 11:28 pm
by tom jupille
That sounds about 10X high. I'd try cutting it down by successive factors of 3 and see what happens.

Posted: Thu Aug 11, 2005 1:38 pm
by SMC
Goto: Post posted by Mass: Sat May 21, 2005 3:52 am
Post subject: What is the limit Percentage of Buffer in LC-MS/MS

Posted: Mon Aug 22, 2005 10:46 pm
by DrPAP
You can get a wide range of concentration recommendations. The least amount of buffer that works for your application is always best. My rule of thumb is less than 10 mM.
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