septum for SPME

[jiangds06]

Do i need a special septum for SPME?

[dlbenach]

I ran our system with a normal Agilent septum and didn't have any problems. The only thing I did was pre-core them first by taking an old fiber and pushing it through the septum while it was installed in the inlet.

[jiangds06]

Thanks a lot!

I ran our system with a normal Agilent septum and didn't have any problems. The only thing I did was pre-core them first by taking an old fiber and pushing it through the septum while it was installed in the inlet.

[MSCHemist]

At my previous position is was common practice to precore the septa with an old fiber. Otherwise any reasonable grade sept with the dimple in the middle is advisable.

[xeim]

Do i need a special septum for SPME?

Yes you do. You have to use pre-drilled septum in your analytical equipment (for example in GC). Las time I "succeed" to break 3 of the fibers in 2 weeks just because my septum in the GC wasn't pre-drilled.

[FCCHEESE]

It depends upon how many samples your are running. Assuming your using a automated injector its best to use a merlin microseal for the long term, these are very realiable and we have very good reproducibility.