How to use Sephadex LH-20 in column chromatography?

[gbala87]

Can anyone help me out with Sephadex LH-20 in column chromatography?
How to pack this material in open column and how to load the sample which is to be purified?
Thanks in advance.

[skunked_once]

https://www.gelifesciences.com/gehcls_i ... 235244.pdf

You're welcome. Google is your friend.

[gbala87]

Thank you for the doc. Can you tell me how to calculate bed volume in simple terms? It would be grateful.

[skunked_once]

Bed volume refers to the volume of packing material in your column. Look up the formula for calculating volume based on column length and radius, or is it diameter? I never remember these things so I always look them up to get it right