Chromatography Forum

Posted: **Fri Sep 27, 2013 8:42 pm**

Do i need a special septum for SPME?

Posted: **Fri Sep 27, 2013 8:58 pm**

I ran our system with a normal Agilent septum and didn't have any problems. The only thing I did was pre-core them first by taking an old fiber and pushing it through the septum while it was installed in the inlet.

Posted: **Fri Sep 27, 2013 9:56 pm**

Thanks a lot!

I ran our system with a normal Agilent septum and didn't have any problems. The only thing I did was pre-core them first by taking an old fiber and pushing it through the septum while it was installed in the inlet.

Posted: **Wed Oct 02, 2013 1:37 pm**

At my previous position is was common practice to precore the septa with an old fiber. Otherwise any reasonable grade sept with the dimple in the middle is advisable.

Posted: **Fri Oct 04, 2013 9:05 am**

Do i need a special septum for SPME?

Yes you do. You have to use pre-drilled septum in your analytical equipment (for example in GC). Las time I "succeed" to break 3 of the fibers in 2 weeks just because my septum in the GC wasn't pre-drilled.

Posted: **Fri Oct 11, 2013 1:16 pm**

It depends upon how many samples your are running. Assuming your using a automated injector its best to use a merlin microseal for the long term, these are very realiable and we have very good reproducibility.

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septum for SPME

septum for SPME

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