Page 1 of 1
How to use Sephadex LH-20 in column chromatography?
Posted: Fri Sep 27, 2013 1:48 pm
by gbala87
Can anyone help me out with Sephadex LH-20 in column chromatography?
How to pack this material in open column and how to load the sample which is to be purified?
Thanks in advance.
Re: How to use Sephadex LH-20 in column chromatography?
Posted: Fri Sep 27, 2013 2:10 pm
by skunked_once
Re: How to use Sephadex LH-20 in column chromatography?
Posted: Sat Sep 28, 2013 11:18 am
by gbala87
Thank you for the doc. Can you tell me how to calculate bed volume in simple terms? It would be grateful.
Re: How to use Sephadex LH-20 in column chromatography?
Posted: Mon Sep 30, 2013 1:40 pm
by skunked_once
Bed volume refers to the volume of packing material in your column. Look up the formula for calculating volume based on column length and radius, or is it diameter? I never remember these things so I always look them up to get it right
