Chromatography Forum

Can anybody guide me HPLC method for 2-Trifluoromethylphenothiazine

Here is a good start

Practical HPLC method development 2ed - Snyder, Kirkland & Glajch

This book outlines method development very well.

Looking at your compound I would suggest using a phosphate pH buffer at 2.5 and trying agradient from 95%buffer 5% acetonitrile to 80% acetonitrile and see where your compound elutes. It looks like you may have a pka around 4 or 5 so you may want to stay away from those pH for you mobile phase.

Of course if you know your compound is only soluble at 50% acetonitrile or something then you will start at 50% acetonitrile instead of 5%.

Good luck

We have this interactive guide which asks you bunch of questions related to your analyte and then gives you recommendation. It might not look like your molecule but you ended up in the right place if you know properties of your molecule (you need to know basic chemistry)

http://www.sielc.com/MethodDevelopment_ ... dType.html