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Liquid chromatography capacity factor
Posted: Thu Aug 04, 2005 6:42 pm
by ashvin
Hi
At presently I have just joined analytical method development,
I am confused how to calculate capacity factor practically.
Thank you in advance
Ashvin
Posted: Thu Aug 04, 2005 7:29 pm
by tom jupille
1. Measure the retention time of your peak and the dead time of your column.
2. Divide retention time by dead time and subtract 1
k' = (tR/t0) - 1
Posted: Thu Aug 04, 2005 9:13 pm
by ashvin
Thanx ,
But how to calculate columne dead volume?
Ashvin
liquid chromatography
Posted: Thu Aug 04, 2005 9:15 pm
by ashvin
How to calculate column dead time
Posted: Fri Aug 05, 2005 12:40 am
by tom jupille
You don't calculate it, you measure it, just like you measure retention time. If you don't see a "solvent front" on your chromatogram, try injecting pure acetonitrile or methanol (I'm assuming you're using reversed phase here) and look for a small disturbance on the baseline.