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UV absorbance in blank sample

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UV absorbance in blank sample

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jiangds06
in base line, it shows huge absorbance in 225 nm. How can get rid of it?

Re: UV absorbance in blank sample

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HPLCaddict
More details, please!
What do you mean by "blank sample"? is it a solvent injection or a blank run without injection?
What does "huge absorbance" mean? A single peak? Multiple peaks? t0 noise? Baseline drift?

Re: UV absorbance in blank sample

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Gerhard Kratz
What flow, what mobile Phase, what HPLC System, compound dissolved in??
Yes, we Need more Details.
Gerhard Kratz, Kratz_Gerhard@web.de
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