Liquid chromatography capacity factor

[ashvin]

Hi
At presently I have just joined analytical method development,
I am confused how to calculate capacity factor practically.

Thank you in advance
Ashvin

[tom jupille]

1. Measure the retention time of your peak and the dead time of your column.
2. Divide retention time by dead time and subtract 1

k' = (tR/t0) - 1

[ashvin]

Thanx ,
But how to calculate columne dead volume?
Ashvin

[ashvin]

How to calculate column dead time

[tom jupille]

You don't calculate it, you measure it, just like you measure retention time. If you don't see a "solvent front" on your chromatogram, try injecting pure acetonitrile or methanol (I'm assuming you're using reversed phase here) and look for a small disturbance on the baseline.