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dmso for solvent

Posted: Fri Sep 06, 2013 9:14 pm
by gerapas
On Sunday i will go to the lab to run a new method for us. today i took a quick glance at it and i saw that the solvent is DMSO/MEOH 90:10. I don't recall the analyte's name but the m.phase is a typical buffer/ACN isocratic and a C18 100x4,0 column. As i have never seen such a solvent before, i thought i would ask the forum for any recomendations (something is likely to bother me this Sunday?), because it is the first time we are doing this method, and it's Sunday, and noone would like me to fail.

Re: dmso for solvent

Posted: Sat Sep 07, 2013 8:15 pm
by HPLCaddict
A typical silica-based C18 column will have no problems with DMSO as sample solvent. Be warned, though, that due to its high viscosity you might see a significant pressure pulse upon injection.

Re: dmso for solvent

Posted: Sun Sep 08, 2013 8:52 pm
by gerapas
All went fine. Thank you hplcaddict so so so much for answering all of my (silly?) questions. you are gold :D