[SOLVED] Agilent Chemstation B03.01 - Signalwindow and Int..

[Perter]

Hi there,

I worked as a guest in a foreign lab for several months to determine the amount of cyclic GMP in different samples with an HPLC system. In the end (means: before data analysis) I ran out of time and just installed the Agilent Chemstation software (B03.01) on my laptop and copied the raw data files.
Back in my homelab I see that the software has problems with displaying the UV signals properly. When I try to zoom in, I see a totally different part of the signal; that was not what I chose. I also tried to set the view via the graphics settings. The X values are OK, but Y not. When I type Y = 2000 mAU I see just 18 mAU. Additionally it's not possible to set manual integration peak baselines. I always get the message: "not enough points to integrate". I do not trust the automatic integration and I do not like the results of automatic baseline / peak finding.
My OS is Windows 7 32 bit.
I also installed the software on a Win7 64 bit system. There the software does not even start (.NET Framework Error 0x800700B). The last thing I tried was installing it on a virtual XP 32 bit system. Same problems with zoom and integration.

An other try was to use OpenChrom (0.5 and 0.6). I installed the Agilent converter to open the files/folderes (*.D), but: "the chromatogram couldn't be loaded". The same thing happens when I export files from Chemstation in *.aia and try to open it then with OpenChrom.

Any Ideas? I do not care about which software I use. I only need to define peaks and get integration values. That's it :/

Thanks for reading
Perter

[Perter]

SOLVED.

I realized that it only zoomed to e.g. 4.0 - 5.0 even if I typed 4.2 to 5.5 in the signal options.
I had to change the decimal point / comma settings in the windows 7 system settings ...

Now everything including the mouse zoom and the manual integration is possible.

[VasaCh]

I had the same problem - many thanks for your advice.