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analysis of polyacrilic acid in ophthalmic solution
Posted: Thu Aug 29, 2013 5:01 pm
by William Jimenez
have some method of analysis of polyacrylic acid in ophthalmic solution?
HPLC or GC method?
Re: analysis of polyacrilic acid in ophthalmic solution
Posted: Fri Aug 30, 2013 3:06 pm
by Consumer Products Guy
have some method of analysis of polyacrylic acid in ophthalmic solution?
HPLC or GC method?
Too big for GC.
Re: analysis of polyacrilic acid in ophthalmic solution
Posted: Thu Sep 05, 2013 3:16 am
by Meerkat
have some method of analysis of polyacrylic acid in ophthalmic solution?
HPLC or GC method?
*just get you a simple gel column with H+ cation exchange capability and use USP as the mobile phase on an LC.
Re: analysis of polyacrilic acid in ophthalmic solution
Posted: Tue Sep 17, 2013 4:18 pm
by William Jimenez
Thank you.
I have some questions:
-such as the treatment of the sample?
-not be filtered with 0.45um filter pore size when diluted with water.
have you any application or description of the method?
Re: analysis of polyacrilic acid in ophthalmic solution
Posted: Tue Sep 17, 2013 4:53 pm
by Vlad Orlovsky
You can use Primesep C column in anion-exclusion mode similar to algininc acid, which is also an acidic polymer:
http://www.sielc.com/Compound-Alginic-Acid.html
This method is validated in two companies.
Re: analysis of polyacrilic acid in ophthalmic solution
Posted: Wed Sep 18, 2013 9:39 pm
by MestizoJoe
Vlad's suggestion looks fantastic based on the link provided.
If you have an SEC column handy you may want to try that too since your large polymer molecule will elute first, followed by smaller molecules. if you have other UV active large molecules in your solution you will need to make sure they don't co-elute.
Re: analysis of polyacrilic acid in ophthalmic solution
Posted: Wed Sep 18, 2013 9:54 pm
by Vlad Orlovsky
here is an article on analysis of aginic acid in Oral suspension using Primesep C column. I know at least two other places which use similar method
http://sphinxsai.com/s_v2_n2/PT_V.2No.2 ... -1638).pdf
Acrylic acid has pKa close to alginic acid. Algininc acid is a polymer with the wide range of MW, but it still elutes as a relatively narrow peak.