Chromatography Forum

Hi, can anybody teach me how to create or make calibration from the galaxie chromatogram using standard? i will have 7 different standards types of fatty acids and need to creat calibration so i can have final result (concentration) of my samples

Hello,

It is very well explain in the User's manual and inthe on-line help of Galaxie.

The calibration curve in Galaxie is a file created separately from the data file (*.calb) (thus you can use it for another data file from another method). But it has to be created from a method first.
In the method, you have to set up the calibration part accordingly to the manual. It will then create one calibration curve ( in a same *.calb file) per component that you will be able to see in the calibration view (you have 3 views in Galaxie : Data, System and Calibration)
It is complicated to explain and teach you directly, so you will have to learn yourself

Good luck

Best regards

BTW, you can find some e-learning modules in DriverCentral from Persistent Systems
https://www.drivercentral.net/product.h ... 35424701b3
In the FAQ part, there a couple of modules for simple operation. Calibration is not detailed, but it will at least show where it is located in Galaxie

Best regards

If you have resolved your mix, on the first step is identify each peak. Open you chromatogram, go to method section, go to integration events and obtain the integrations for the each peak of your interest. After that, go to peak identification and rigth click . Choice "initialize from chromatogram", put the names for each peak. and integrate F5, after this, on the chromatogram are the names. Now go to calibration section and define the calibration type, levels of calibration, units, give a name for your calibration file. Click on initialize from ID tables. Now put the concentraction value for each peak and level. Go to save, and click on the save "cromato method". This, save the changes on the method analysis. Close your method analysis, if it is open, this refresh the changes on your method.

Now open your chromatograms of each level calibration, go to reproces an click, in the new window, on the chromatogram, select the first chromatogram, normally the lowest concentratation, select your method analysis wich contain your calibration file. On the sample type select "standard", on calibration mode select "clear old points", now select calibration level, normally 1. Repeat all of this with the others chromatograms, but for all of them select in calibration mode "add". Close your reprocess window.

If all is OK, go to calibration section, on the bottom of your main page and open your calibration file. You can observe the calibrations curves. At this moment, your method analysis is now a cuantitation methos, when you analyze samples you can obtain the concentratio of each compound.

I hope yhis help.

E.M. Calidad Analítica S.A. de C.V.
emcasa_cv@hotmail.com