Chromatography Forum

Hi,
Im an Amateur chromatographer and i was trying to separate enantiomers of a drug on a lux chiral column by using polar organic solvent chromatography and i tried many permutations and combinations of the organic solvents, and at some point i was able to achieve a nice peak resolution with a symmetric peak shape only after using hexane as a modifier.
Now my question is whether can we use hexane as a modifier in POSC? and let me tell u that there are no miscibility issues in there and i used only 10% of Hexane and the mobile phase contains MeCN and MeOH

Use hexane and be happy.
Best Regards

First of all.. thanks fr the reply
Danko
and am using Hexane and its good, but i need a help..... is there any method which uses Hexane and MeCN at a time in a mobile phase for HPLC

I'm sure there is/are.

I tried a lot for the literature but couldn't find a single paper which uses both Hexane and MeCN as a mobile phase.
But if u have one can u pls provide me the link Danko... thanks in advance

Use iso-hexane and not n-hexane due to SHE issue.
And iso-hexane is much cheaper than n-heptane.
It works very well.

Hi sandy1989,

I can't provide a link to such a method and unfortunately I don't have the time to do a search.
But I can not - in my wildest fantasies - imagine that there aren't any instances.

best Regards

Well Thanks for ur valuable time and suggestion danko....

Just for a start:
http://bit.ly/15BBg4x

I don't have access to the fulltext, but "The effects of trifluoroacetic acid (TFA) and n-hexane concentration in an acetonitrile (MeCN) mobile phase were investigated" sounds like something you might be looking for.

Thanks HPLCaddict and I have this article already but even I forgot that ,... it was helpful not totally but as u said its a Start... .

Csaba:

Can you please explain what you meant by this: "Use iso-hexane and not n-hexane due to SHE issue."

Thanks!!

SHE is Safety Health Environment.....

SHE = Safety Health Environment - common aberration in the industry. n-Hexane is cancerogenic and you have to have a good reason to use it otherwise it is NO.

Hi Csaba ,

I couldn't locate any Material Safety Data that mentions n-hexane is carcinogen.

OSHA , NTP and IARC classifies at not carcinogen.
NFPA Rating for Health=2

Hi follow the link below

http://www.atsdr.cdc.gov/toxfaqs/tf.asp?id=392&tid=68

its not carcinogenic but inhalation causes nerve damage and paralysis of the arms and legs