Chromatography Forum

Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/

HPLC analysis of tea catechins

http://www.chromforum.org/viewtopic.php?t=23252

Page 1 of 1

HPLC analysis of tea catechins

Posted: Wed Aug 21, 2013 10:46 am
by murugesh
I am using Epigallocatechin (EGC), Epigallocatechingallate(EGCG), Epicatechingallate(ECG) and Epicatechin(EC) as standards in HPLC based quantification of these catechins in tea samples. Is it possible to get same peak area for same concentration of all the four compounds or it will depend on the extinction coefficient of compounds. I am using isocratic elution, mobile phase is water:methanol:acetic acid(73:23:1). Coloumn is oyster BDS premium C18 (300mm X 4.6 mm), coloumn temperature: 313K.

Waiting for the kind response from experts
Murugesh

Re: HPLC analysis of tea catechins

Posted: Wed Aug 21, 2013 2:29 pm
by tom jupille
It will depend on the extinction coefficient.
All times are UTC
Page 1 of 1
Powered by phpBB® Forum Software © phpBB Limited
https://www.phpbb.com/