wavelength accuracy

[carpenh]

I need to use a detection wavelength of 774 nm. I plan to use a DAD. Can I or should I do some kind of wavelength accuracy. If so, how can this be accomplished?

[Meerkat]

use the "UV-Vis accuracy precision check kit", it's a commonly used system suitability check--and also estimates the stray light interference--the kits are actually quite good; they are easily available online by the typical search/find procedures.
I use it once per quarter year on our shimadzu uv-vis device.
bon chance!

[Gerhard Kratz]

Are you sure about 774 nm??? Or do you mean 774 THz? 774nm would be extraterrestical sun light.

[juddc]

774 nm sounds reasonable to me. My PDA has a range from 190-800 nm, which is typical. Are you playing with something like this:
http://www.licor.com/bio/products/reage ... sEster.jsp ?

Erbium perchlorate is what I've used in the past, though it only is typically used up to ~522 mn, I believe. Holmium oxide has a peak at 641-nm. I don't know of much that goes beyond that.

How critical is this?

[tom jupille]

How critical is this?

1. UV or visible absorbance spectra in solution usually do not have a great deal of fine structure.
2. the bandpass on HPLC detectors is usually fairly wide.
3. Even the USP and EP allow +/- 3 nm error in their default adjustment limits.
4. In any case, you are running your calibrators and samples on the same instrument so any error would apply equally to both.

Conclusion: unless the compound has a feature-rich spectrum, I usually don't worry about wavelength accuracy very much.