Help with Chemstation

[Apple_insci]

It seems I chose the wrong reference wavelength. So my peaks are negative. Does anyone know if I can change the reference wavelength and reprocess the data or can I use "isoabsorbace plot" function or other function to somehow correct it?

[pawan ratra]

Yes you can extract at any wavelength and with any refernce wavelength. But the data should be acquired with all data points and in complete uv range. Can you please describe the complete signal details for acquiring data.

[Tequila]

Dear pawan ratra,

I work with Chemstation but not a good detector because it is a variable wavelength detector. I do not know which is your detector but I think in the worst situation that could a fixed wavelength detector. You coul work with a standard solution and a spectrophotometer where you could see the spectrum and select the best wavelength.
Good luck,

Diego Delmonte

[Carlos Teixeira]

Dear Apple_insci

If You work a reference wavelenght in ChemStation, so you use a DAD detector, isn't? So you can determine the DAD spectra of your peaks, and the peak of your analyte. It is easy, but if you have any problem... contact the Agilent Local Office in you country or send us notice.

Have good elutions!!!

Carlos Teixeira