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Mobile phase pH (MeCN/water)
Posted: Wed Aug 14, 2013 10:05 pm
by DJ
Does anyone have concise list for various buffers, acids, bases used in chromatography, and their pKa is MeCN/water mixes?
Specifically, i'm interested in pH as a function of MeCN% for phosphoric acid, TFA, diethylamine
Re: Mobile phase pH (MeCN/water)
Posted: Thu Aug 15, 2013 6:01 am
by Gerhard Kratz
Many years ago when I worked for Waters we did a nice presentation on buffers, anorganic, organic, biological, volatile buffers. We wanted to teach customers to use volatile buffers instead of sodium Phosphate buffers. The list of buffers was completed with UV spectra to Show the UV cut off.
PH will Change when you add organic modifiers, but so far I know there is no list available or presentation. If I'm wrong I would like to get such a presentation as well!
Please ask your contact at Waters if you can get the "old" buffer presentation. That may help you a Little bit.
Good luck.
Re: Mobile phase pH (MeCN/water)
Posted: Thu Aug 15, 2013 6:19 am
by vahur
Maybe you can find help from here:
http://tera.chem.ut.ee/~ivo/HA_UT/
Re: Mobile phase pH (MeCN/water)
Posted: Thu Aug 15, 2013 9:14 am
by Gerhard Kratz
My god, who did all this work? Excellent source. Thanks
Re: Mobile phase pH (MeCN/water)
Posted: Thu Aug 15, 2013 1:45 pm
by vahur
Analytical Chemistry department in Tartu University
Re: Mobile phase pH (MeCN/water)
Posted: Thu Aug 15, 2013 4:16 pm
by juddc
I second Gerhard's compliment - Excellent!
Re: Mobile phase pH (MeCN/water)
Posted: Fri Aug 16, 2013 6:46 pm
by shaun78
Wow!
Who got to run all the DFT calculations? pretty aggressive method and basis set.