Page 1 of 1
which fragment ion should be used for quantification
Posted: Tue Aug 06, 2013 10:40 pm
by jiangds06
generally base peak, or the second largest peak?
Re: which fragment ion should be used for quantification
Posted: Wed Aug 07, 2013 12:29 pm
by rb6banjo
I usually choose one that is unique and hopefully quite larger. A unique fragment makes most of the other stuff you don't care about vanish and a large one gets you the sensitivity you want. You should also monitor some others that are characteristic to your analyte so that you don't identify a coeluter as your analyte. The ratios of the fragments should be characteristic of your analyte. Generally, even isomers have different ratios of the fragments generated.
Good luck!
Re: which fragment ion should be used for quantification
Posted: Wed Aug 07, 2013 10:35 pm
by jiangds06
make sense. thanks.
I usually choose one that is unique and hopefully quite larger. A unique fragment makes most of the other stuff you don't care about vanish and a large one gets you the sensitivity you want. You should also monitor some others that are characteristic to your analyte so that you don't identify a coeluter as your analyte. The ratios of the fragments should be characteristic of your analyte. Generally, even isomers have different ratios of the fragments generated.
Good luck!