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column for very high pressure chromatography

http://www.chromforum.org/viewtopic.php?t=2310

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column for very high pressure chromatography

Posted: Mon Aug 01, 2005 5:37 pm
by lotusw
Hello,
I currently work with a method HPLC which necessite to use a column RP C18 250*4.6mm with a flow of 2.5 ml/min and a great quantity of water (80%). Of course the pressure is high (around 4000 PSI). I don't have the choice, the method is imposed by USP. I would like to know if there are columns having its caracteristiques while having a lower pressure.
Thanks.

Posted: Mon Aug 01, 2005 7:35 pm
by Klaus I.
Search with Google for "Chromolith Performance" from Merck KGaA.

High Pressure HPLC

Posted: Tue Aug 02, 2005 4:19 pm
by Sallybeetle
What is the USP column listing, L1? If resolution factor is not an issue, then you might also try using the upper particle size limit.

Posted: Tue Aug 02, 2005 8:51 pm
by Uwe Neue
The original column was probably a 10 micron column. You should therefore look for a 10 micron column then.

The USP also allows you to change the column length, as long as you can meet the other criteria of the assay.
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