Hi everyone,

I need to have any ideas about developing a method with several vitamines including B12 and its impurity.

I developed a method to quantify B1, B6, B12 but just realise that hydroxocobalamine coelute with B12.
Is there a way to separate cyanocobalamine (B12) from hydroxocobalamine ?

I don't find a way to separate them on a UPLC method. I am working on a simple purospher star C18 and changing mobile phase does not help.

Thank you for your help.