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Problem of solvent during liquid liquid extraction

Posted: Tue Jul 16, 2013 3:41 pm
by KJO_35
Hi everybody,

In order to quantify different pesticides by GC/MS, I'm currently using a liquid-liquid extraction step with hexane for the transfer of pesticides from an aqueous solution (HBSS). This method works pretty well regarding the extraction of atrazine, simazine, propazine and its metabolite DEA with their respective logP value 2,75 - 2,2 - 2,9 - 1,51. But regarding the other metabolites DIA and DACT with logP value 1,01 and -0,1 respectively, the solvent is not appropriate.

Does anyone have an idea of solvent that could be used for the extraction of these 2 weakly lipophilic compounds ?

Thank you very much for your help!

Re: Problem of solvent during liquid liquid extraction

Posted: Tue Jul 16, 2013 3:47 pm
by Don Shelly
Since they are so polar, you will need a polar solvent. Dry saturating your sample with magnesium sulfate and shaking out with acetonitrile. Centrifugation will separate the water from the solvent. (ACN on top)