Never-ending problems on the Perkin Elmer FX-15
Posted: Mon Jul 15, 2013 12:49 am
Has anyone experienced continuous problems on the PE FX-15 UHPLC. A brand new machine, one pulse dampener has been replaced... and others....
But right now I am running taurine std's pre-column derivatized by 1-fluoro-2,4 dinitrobenzene to form a UV active analyte. Mobile phase is 80/20 0.01 M phosphate buffer pH 6/ACN. Flow rate has varied from 1 to 2 ml/min from method opt.
The baseline is completely out of whack as you can see
. What is going on. Thank you so much to everyone.
-Mike
But right now I am running taurine std's pre-column derivatized by 1-fluoro-2,4 dinitrobenzene to form a UV active analyte. Mobile phase is 80/20 0.01 M phosphate buffer pH 6/ACN. Flow rate has varied from 1 to 2 ml/min from method opt.
The baseline is completely out of whack as you can see
-Mike
