hplc calibration

[AHSJ1269]

in HPLC calibration wave length accuracy

how i determine wave length accuracy of detector at lower uv wave lenghts i.e. 210 nm , 205 nm

[tom jupille]

You don't. Almost any signal you see in that region is "end absorbance" which continues to increase as you go to shorter wavelengths (i.e., hard to find anything that has a true absorbance maximum in that region).

[lmh]

I sort-of feel that wavelength calibration is one of the less useful calibrations we do anyway, at least in quantitative work.

Most things have fairly broad maxima, so if you're out by a few nm, it won't make any difference. And in any case, if the standards and samples are both measured at the same wavelength, the actual exact wavelength used probably doesn't matter. If you're comparing wavelength maxima to expected values as part of a strategy for identifying things, that of course is a whole different situation.