Advertisement

Using stabilized THF for GPC

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
I'm setting up a new preparative GPC using an RI detector and I'm wondering if I should be using stabilized THF. Since I'm not using a UV-Vis detector, I don't have to worry about BHT interference. The biggest problem I'm having is recovering fractions from the GPC since drying the unstabilized THF produces peroxides. Any help or advice would be greatly appreciated!
At my last position, we used stabilized THF, mainly for safety reasons - our safety department didn't want to take a chance with peroxides forming in solution.

Also the price difference is not that much, I would suggest you use stabilized THF if you are going to be recycling the eluate. Your safety is worth more than a few bucks saved!
BHolmes

Any problem worthy of attack, proves its worth by hitting back...never give up!
2 posts Page 1 of 1

Who is online

In total there are 16 users online :: 1 registered, 0 hidden and 15 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Amazon [Bot] and 15 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry