HPLC-UV to ESI or APCI
Posted: Mon May 20, 2013 10:05 pm
Hello,
Could someone please help me in developing a method on triple quadrupole mass spectrometer. I have a method developed on HPLC-UV and the solvent system used was hexane/Isopropanol +0.1% triethylamine (90:10), and the analytes are basic in nature (Pka around 10). I am planning to develop a method on mass spec. To my knowledge it's difficult to see an ESI spectrum using hexane as a solvent. I did a trial on ESI and APCI by direct injection using the same (as given above) combination of solvents, but I wasn't able to see any peaks. The coulmn is compatible with solvents such as alkanes, alcohols (methanol, ethanol and Isopropanol), and acetonitrile. Could someone please suggest me to develop a method on mass spec. I would be happy to provide more information if needed. Thanks in advance for your reply.
Thanks,
Sravan Mansani.
Could someone please help me in developing a method on triple quadrupole mass spectrometer. I have a method developed on HPLC-UV and the solvent system used was hexane/Isopropanol +0.1% triethylamine (90:10), and the analytes are basic in nature (Pka around 10). I am planning to develop a method on mass spec. To my knowledge it's difficult to see an ESI spectrum using hexane as a solvent. I did a trial on ESI and APCI by direct injection using the same (as given above) combination of solvents, but I wasn't able to see any peaks. The coulmn is compatible with solvents such as alkanes, alcohols (methanol, ethanol and Isopropanol), and acetonitrile. Could someone please suggest me to develop a method on mass spec. I would be happy to provide more information if needed. Thanks in advance for your reply.
Thanks,
Sravan Mansani.